TetR repressor-based bioreporters for the detection of doxycycline using Escherichia coli and Acinetobacter oleivorans
H Hong, W Park - Applied microbiology and biotechnology, 2014 - Springer
Tetracycline (TC)-sensing bioreporters using green fluorescent protein (GFP) were
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible
promoter, tetH promoter, and a TetR repressor of the pAST2 plasmid recovered from sludge
were used to construct plasmid-based and chromosome-based bioreporters. Two host
plasmids with a broad range, pRK415 and pBBR1MCS2, and three randomly chosen
chromosomal sites were used to create the reporter strains. Although the copy numbers of …
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible
promoter, tetH promoter, and a TetR repressor of the pAST2 plasmid recovered from sludge
were used to construct plasmid-based and chromosome-based bioreporters. Two host
plasmids with a broad range, pRK415 and pBBR1MCS2, and three randomly chosen
chromosomal sites were used to create the reporter strains. Although the copy numbers of …
TetR repressor-based bioreporters for the detection of doxycycline using Escherichia coli and Acinetobacter oleivorans.
HHR Hong HyeRim, PWJ Park WooJun - 2014 - cabidigitallibrary.org
Tetracycline (TC)-sensing bioreporters using green fluorescent protein (GFP) were
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible
promoter, tetH promoter, and a TetR repressor of the pAST2 plasmid recovered from sludge
were used to construct plasmid-based and chromosome-based bioreporters. Two host
plasmids with a broad range, pRK415 and pBBR1 MCS2, and three randomly chosen
chromosomal sites were used to create the reporter strains. Although the copy numbers of …
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible
promoter, tetH promoter, and a TetR repressor of the pAST2 plasmid recovered from sludge
were used to construct plasmid-based and chromosome-based bioreporters. Two host
plasmids with a broad range, pRK415 and pBBR1 MCS2, and three randomly chosen
chromosomal sites were used to create the reporter strains. Although the copy numbers of …
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